Journal
/
Immunology and Infection
/
Studying ER Stress and UPR Activation During HIV-1 Infection in T-Cells
/
shRNA-Mediated Gene Knockdown בתאי HEK-293T ו- Jurkat J6, טיפול ב- ER Stress Inducer, וניתוח השפעתו על שכפול HIV-1
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
shRNA-Mediated Gene Knockdown in HEK-293T and Jurkat J6 Cells, Treatment with ER Stress Inducer, and Analyzing its Effect on HIV-1 Replication
Please note that all translations are automatically generated. Click here for the English version.

shRNA-Mediated Gene Knockdown בתאי HEK-293T ו- Jurkat J6, טיפול ב- ER Stress Inducer, וניתוח השפעתו על שכפול HIV-1

עברית

Automatically Generated

50 Views

•

03:08 min

•

June 14, 2024

DOI:

10.3791/201940-v

DOI:
10.3791/201940-v

•

03:08 min
June 14, 2024

•

14 Views
, ,
1National Centre for Cell Science,SP Pune University Campus

Explore More Videos

ShRNA-mediated Gene KnockdownHEK-293T CellsJurkat J6 CellsER Stress InducerHIV-1 ReplicationTransfection MixLuciferase SubstrateGFP FluorescenceLentiviral SupernatantPuromycin SelectionThapsigargin TreatmentCytotoxicity AnalysisMTT AssayImmunoblottingHSPA5 ExpressionP24 ELISA

Read Article

Cite this Article

shRNA-Mediated Gene Knockdown in HEK-293T and Jurkat J6 Cells, Treatment with ER Stress Inducer, and Analyzing its Effect on HIV-1 Replication. J. Vis. Exp. (Pending Publication), e201940, doi:10.3791/201940 (2024).

Download .ris file

Copy

Share Video

.

Copy