Journal
/
Immunology and Infection
/
Isolation and Characterization of Antigen-Specific T Cells in Mouse Lungs
/
توليد تعليق أحادي الخلية من أنسجة الرئة لتحديد مجموعات الخلايا التائية النادرة الخاصة بالمستضد
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Generating Single-Cell Suspension from Lung Tissue for Identifying Rare Antigen-Specific T-Cell Populations
Please note that all translations are automatically generated. Click here for the English version.

توليد تعليق أحادي الخلية من أنسجة الرئة لتحديد مجموعات الخلايا التائية النادرة الخاصة بالمستضد

العربية

Automatically Generated

45 Views

•

03:21 min

•

July 19, 2024

DOI:

10.3791/201919-v

DOI:
10.3791/201919-v

•

03:21 min
July 19, 2024

•

5 Views
, ,
1Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology,Massachusetts General Hospital, 2Division of Immunology,Boston Children’s Hospital, 3Harvard Medical School, 4Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital

Explore More Videos

Single-cell SuspensionAntigen-specific T-cell PopulationsCD45 AntibodyLiberase Stock SolutionAminoguanidine Stock SolutionEHAAMicrofuge TubesDNase I DigestionCentrifugeFc Block Solution

Read Article

Cite this Article

Generating Single-Cell Suspension from Lung Tissue for Identifying Rare Antigen-Specific T-Cell Populations. J. Vis. Exp. (Pending Publication), e201919, doi:10.3791/201919 (2024).

Download .ris file

Copy

Share Video

.

Copy