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Developing 2D Monolayer Cultures from Bovine Intestinal Organoids
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שימוש באורגנואידים רקטליים ו/או רקטליים להקמת תרבית חד-שכבתית דו-ממדית
JoVE Journal
Medicine
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JoVE Journal Medicine
Using Bovine Ileal and/or Rectal Organoids for Establishing 2D Monolayer Culture
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שימוש באורגנואידים רקטליים ו/או רקטליים להקמת תרבית חד-שכבתית דו-ממדית

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03:23 min

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June 14, 2024

DOI:

10.3791/201855-v

DOI:
10.3791/201855-v

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03:23 min
June 14, 2024

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2 Views
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1Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine,Washington State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Washington State University

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Keyword Extraction:BovineIlealRectalOrganoids2D MonolayerCultureExtracellular MatrixCulture InsertCell SuspensionImmunofluorescent StainingScanning Electron MicroscopyMicrovilliAdherence JunctionsGoblet Cells

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Dykstra, G. D., Kawasaki, M., Ambrosini, Y. M. Using Bovine Ileal and/or Rectal Organoids for Establishing 2D Monolayer Culture. J. Vis. Exp. (Pending Publication), e201855, doi:10.3791/201855 (2024).

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