/

/

/

Трансфекция и сбор аденоассоциированных вирусных векторов с использованием клеток HEK293T для генной терапии

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Transfection and Harvesting Adeno-Associated Virus Vectors Using HEK293T Cells for Gene Therapy

Please note that all translations are automatically generated. Click here for the English version.

Трансфекция и сбор аденоассоциированных вирусных векторов с использованием клеток HEK293T для генной терапии

136 Views

•

04:00 min

•

May 03, 2024

DOI:

10.3791/201730-v

DOI:

10.3791/201730-v

•

04:00 min

May 03, 2024

•

12 Views

Ting Zhang¹, Vinitha Dhamotharan¹, Yu Xiao^1,2, Sydne Ballengee^1,3, Jill Pollon^1,4, George K. Gittes¹

¹Division of Pediatric Surgery, Department of Surgery, Children’s Hospital of Pittsburgh,University of Pittsburgh School of Medicine, ²North Allegheny High School, ³Wright State University Boonshoft School of Medicine, ⁴College of Engineering,University of Michigan

Explore More Videos

Transfection HEK293T Cells Adeno-Associated Virus (AAV)Gene Therapy DMEM High-glucose Media PEI Solution Centrifugation AAV Lysis Buffer PEG Sodium Chloride Solution Ultracentrifuge Iodixanol Gradients AAV Dialysis Buffer Downstream Purification Cell Factory

Read Article