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Construction à grande échelle de plasmides UAS-cDNA/ORF par assemblage modulaire basé sur CRISPR (CRISPRmass)
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02:25 min
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May 17, 2024
DOI:
1Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 2Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 3Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 4Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
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Xu, S., Chen, M., Chen, G., Luo, S., Xue, W., Liu, X., Wang, J., Wei, P. Large-Scale Construction of UAS-cDNA/ORF Plasmids by CRISPR-Based Modular Assembly (CRISPRmass). J. Vis. Exp. (Pending Publication), e201691, doi:10.3791/201691 (2024).
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