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Journal
/
Neuroscience
/
Enhancing Peripheral Nerve Regeneration with nsPEF-Stimulated Schwann Cells
/
Preparing Cryopreserved RSC96 Cells for Nanosecond Pulsed Electric Field Stimulation
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparing Cryopreserved RSC96 Cells for Nanosecond Pulsed Electric Field Stimulation
Preparing Cryopreserved RSC96 Cells for Nanosecond Pulsed Electric Field Stimulation
Enhancing Peripheral Nerve Regeneration with nsPEF-Stimulated Schwann Cells
DOI:
10.3791/201659-v
•
02:10 min
•
May 03, 2024
•
Jiahang Han*
1,2
,
Zewei Wang*
1,2
,
Yanzhao Dong*
1
,
Xiaodi Zou
,
Haoyu Wang
2
,
Yonggang Chen
,
Sahar Ahmed Abdalbary
,
Tian Tu
,
Hui Lu
1
Department of Orthopedics, The First Affiliated Hospital, College of Medicine
,
Zhejiang University
,
2
College of Medicine
,
Zhejiang University
,
3
Department of Orthopedics
,
The Second Affiliated Hospital of Zhejiang Chinese Medical University
,
4
Department of Orthopedic Physical Therapy, Faculty of Physical Therapy
,
Nahda University in Beni Suef
,
5
Plastic & Cosmetic Center, The First Affiliated Hospital, College of Medicine
,
Zhejiang University
Tags
Cryopreserved
RSC96 Cells
Nanosecond Pulsed Electric Field Stimulation
Cell Suspension
Centrifuge
Culture Medium
Cell Density
PBS
Trypsin
EDTA
FBS
Cell Detachment
Cell Suspension
T25 Flask
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