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Journal
/
Immunology and Infection
/
Elucidating T-Cell Kinetics: Methods for Real-Time Migration Analysis
/
CD8+ T-Cell Purification from Mouse Lymph Nodes and Spleen for Kinetic Studies
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
CD8+ T-Cell Purification from Mouse Lymph Nodes and Spleen for Kinetic Studies
CD8+ T-Cell Purification from Mouse Lymph Nodes and Spleen for Kinetic Studies
Elucidating T-Cell Kinetics: Methods for Real-Time Migration Analysis
DOI:
10.3791/201597-v
•
02:20 min
•
May 24, 2024
•
Allison T. Ryan
2
,
Ankit Dahal
2
,
Sana Mir
2
,
Minsoo Kim
2
,
Kihong Lim
2
1
Department of Microbiology and Immunology
,
University of Rochester
,
2
David H. Smith Center for Vaccine Biology and Immunology
,
University of Rochester
Tags
CD8+ T-cell
Lymph Nodes
Spleen
Cell Purification
Cell Isolation
Cell Separation
Negative Selection
Magnetic Beads
Cell Suspension
Cell Strainer
Cell Disruption
Red Blood Cell Lysis
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