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Immunology and Infection
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Сборка частиц Ha-CoV-2 путем котрансфекции HEK293T клеток для количественного определения вариантов SARS-CoV-2
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Assembling Ha-CoV-2 Particles by Cotransfection of HEK293T Cells for Quantification of SARS-CoV-2 Variants
Please note that all translations are automatically generated.
Click here for the English version.
Сборка частиц Ha-CoV-2 путем котрансфекции HEK293T клеток для количественного определения вариантов SARS-CoV-2
DOI:
10.3791/201201-v
•
01:40 min
•
September 08, 2023
•
Linda Chilin
,
Brian Hetrick
,
Yuntao Wu
1
Center for Infectious Disease Research, School of Systems Biology
,
George Mason University
Tags
HEK293T Cells
DMEM
FBS
Penicillin
Streptomycin
Trypan Blue
Cell Counting
Cell Culture
Cotransfection
Viral Particle Assembly
SARS-CoV-2 Variants
Ha-CoV-2 Pseudovirus
Centrifugation
Filtration
Storage
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