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Journal
/
Chemistry
/
Histone Proteomic Analysis and Post-Translational Modification Characterization
/
LC-TIMS-PASEF-ToF MS/MS Method for Proteolytic Histone Peptide Analysis
JoVE Journal
Chemistry
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JoVE Journal
Chemistry
LC-TIMS-PASEF-ToF MS/MS Method for Proteolytic Histone Peptide Analysis
LC-TIMS-PASEF-ToF MS/MS Method for Proteolytic Histone Peptide Analysis
Histone Proteomic Analysis and Post-Translational Modification Characterization
DOI:
10.3791/200870-v
•
02:54 min
•
January 12, 2024
•
Meiby Fernandez-Rojas
,
Cassandra N. Fuller
,
Lilian Valadares Tose
,
Matthew Willetts
,
Melvin A. Park
,
Natarajan V. Bhanu
,
Benjamin A. Garcia
,
Francisco Fernandez-Lima
4
1
Department of Chemistry and Biochemistry
,
Florida International University
,
2
Bruker Daltonics Inc.
,
3
Department of Biochemistry and Molecular Biophysics
,
Washington University School of Medicine
,
4
Biomolecular Sciences Institute
,
Florida International University
Tags
LC-TIMS-PASEF-ToF MS/MS
Proteolytic Histone Peptide Analysis
HPLC
C-18 Column
TIMS ToF MS
Nanoelectrospray Ionization
Collision Energy
LC Gradient
MS-Digest
Protein Prospector
Post-translational Modifications
Propionyl
Acetyl
HeLa S3 Cells
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