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포유 동물 세포에서 RNAi 심사를위한 선교 esiRNA

JoVE Journal
Biology

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JoVE Journal Biology

MISSION esiRNA for RNAi Screening in Mammalian Cells

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포유 동물 세포에서 RNAi 심사를위한 선교 esiRNA

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15:31 min

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May 12, 2010

DOI:

DOI:

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15:31 min

May 12, 2010

•

3 Views

Mirko Theis, Frank Buchholz

¹Max Planck Institute of Molecular Cell Biology and Genetics

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MISSION EsiRNA RNAi Screening Mammalian Cells Gene Expression Control Small Interfering RNAs (siRNAs)Dicer RNA-induced Silencing Complex (RISC)Target MRNA Cleavage Loss Of Gene Function Studies Chemical Synthesis Of SiRNAs Endonucleolytic Cleavage Target-specific Long Double-stranded RNAs RNase III Endoribonuclease-prepared SiRNA Efficacy Of SiRNAs And EsiRNAs Off-target Effects

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