Dergi
/
Biyoloji
/
MISSION esiRNA for RNAi Screening in Mammalian Cells
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
MISSION esiRNA for RNAi Screening in Mammalian Cells

MISSION esiRNA for RNAi Screening in Mammalian Cells

DOI:
10.3791/2008-v

•

15:31 min

•

May 12, 2010

•

,
1Max Planck Institute of Molecular Cell Biology and Genetics

Bölümler

  • 00:00Başlık
  • 01:43Introduction
  • 02:22Use of High-Quality MISSION esiRNA Libraries for RNAi Screening
  • 03:49Optimizing Transfection Prior to Screening
  • 05:28Setting Up the Assay and Preparing for the Primary Screen
  • 09:34The Primary Screen
  • 12:03Secondary Screen of the Selected Genes for Hit Validation
  • 13:40Representative Results Screening for Mammalian Cell Cycle Genes Using MISSION esiRNA
  • 14:43Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here we use a human esiRNA library in a high-throughput screen for genes involved in cell division. We demonstrate how to set up and conduct an esiRNA screens, as well as how to analyze and validate the results.

Etiketler

MISSION EsiRNARNAi ScreeningMammalian CellsGene Expression ControlSmall Interfering RNAs (siRNAs)DicerRNA-induced Silencing Complex (RISC)Target MRNA CleavageLoss Of Gene Function StudiesChemical Synthesis Of SiRNAsEndonucleolytic CleavageTarget-specific Long Double-stranded RNAsRNase IIIEndoribonuclease-prepared SiRNAEfficacy Of SiRNAs And EsiRNAsOff-target Effects

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)

Bacterial Delivery of RNAi Effectors: Transkingdom RNAi

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Analysis of Cell Cycle Position in Mammalian Cells

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Measurement of Heme Synthesis Levels in Mammalian Cells

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Read Article