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Journal
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Developmental Biology
/
Satellite Cell Isolation from Mouse Limb Muscles and Flow Cytometry Analysis
/
형광 활성화 세포 분류에 의한 위성 세포 분리를 위한 마우스 근육 세포 현탁액의 제조
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Preparation of Mouse Muscle Cell Suspension for Isolation of Satellite Cells by Fluorescence-Activated Cell Sorting
Please note that all translations are automatically generated.
Click here for the English version.
형광 활성화 세포 분류에 의한 위성 세포 분리를 위한 마우스 근육 세포 현탁액의 제조
Satellite Cell Isolation from Mouse Limb Muscles and Flow Cytometry Analysis
DOI:
10.3791/200375-v
•
03:36 min
•
July 07, 2023
•
Kamar Ghaibour*
1
,
Joe Rizk*
1
,
Claudine Ebel
,
Tao Ye
,
Muriel Philipps
,
Valérie Schreiber
,
Daniel Metzger
,
Delphine Duteil
1
CNRS, Inserm, IGBMC UMR 7104- UMR-S 1258
,
Université de Strasbourg
Tags
Mouse Muscle Cell Suspension
Satellite Cell Isolation
Fluorescence-activated Cell Sorting
Limb Muscle Dissection
Collagenase Digestion
Red Blood Cell Lysis
Cell Staining
Flow Cytometry
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