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Developmental Biology
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Satellite Cell Isolation from Mouse Limb Muscles and Flow Cytometry Analysis
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Preparation of Mouse Muscle Cell Suspension for Isolation of Satellite Cells by Fluorescence-Activated Cell Sorting
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Developmental Biology
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JoVE Journal Developmental Biology
Preparation of Mouse Muscle Cell Suspension for Isolation of Satellite Cells by Fluorescence-Activated Cell Sorting

Preparation of Mouse Muscle Cell Suspension for Isolation of Satellite Cells by Fluorescence-Activated Cell Sorting

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03:36 min

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July 07, 2023

DOI:

10.3791/200375-v

DOI:
10.3791/200375-v

•

03:36 min
July 07, 2023

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5 Views
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1CNRS, Inserm, IGBMC UMR 7104- UMR-S 1258,Université de Strasbourg

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Mouse Muscle Cell SuspensionSatellite Cell IsolationFluorescence-activated Cell SortingLimb Muscle DissectionCollagenase DigestionRed Blood Cell LysisCell StainingFlow Cytometry

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Ghaibour, K., Rizk, J., Ebel, C., Ye, T., Philipps, M., Schreiber, V., Metzger, D., Duteil, D. Preparation of Mouse Muscle Cell Suspension for Isolation of Satellite Cells by Fluorescence-Activated Cell Sorting. J. Vis. Exp. (Pending Publication), e200375, doi:10.3791/200375 (2023).

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