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Separação de Construções de Ácidos Nucleicos Polimerizados por PAGE

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Separation of Polymerized Nucleic Acid Constructs by PAGE

Separação de Construções de Ácidos Nucleicos Polimerizados por PAGE

142 Views

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02:37 min

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May 12, 2023

DOI:

10.3791/200253-v

DOI:

10.3791/200253-v

•

02:37 min

May 12, 2023

•

1 Views

Alice Sosic¹, Cristina Dal Lago¹, Richard Göttlich², Barbara Gatto¹

¹Department of Pharmaceutical and Pharmacological Sciences,University of Padova, ²Institute of Organic Chemistry,Justus Liebig University Giessen

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PAGE Polymerized Nucleic Acid Constructs Gel Electrophoresis TBE Buffer Denaturing Gel Loading Buffer FAM-labeled Oligonucleotide Bsep 1 G4 TBA Single-stranded TBA Double-stranded TBA Alkylation Adduct Stranded Cleavage DNA Fragmentation Tris Buffer

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