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Biochemistry
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Measuring Ubiquitylated, SUMOylated, and ADP-Ribosylated DNA-Protein Crosslinks
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כתם מערבי של דגימות DNA מעוכלות המכילות קישורים צולבים של DNA-חלבון (DPC)
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Western Blotting of Digested DNA Samples Containing DNA-Protein Crosslinks (DPCs)
Please note that all translations are automatically generated. Click here for the English version.

כתם מערבי של דגימות DNA מעוכלות המכילות קישורים צולבים של DNA-חלבון (DPC)

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241 Views

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03:14 min

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April 21, 2023

DOI:

10.3791/200210-v

DOI:
10.3791/200210-v

•

03:14 min
April 21, 2023

•

1 Views
1Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health

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Western BlottingDNA-protein CrosslinksDPCsSDS-PAGECoomassie Blue StainUbiquitylationSUMO-1SUMO-2SUMO-3ADP RibosylationTOP1TOP2 AlphaTOP2 BetaCamptothecinAdipocyteFormaldehydePARylationPARG Inhibitor

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Sun, Y. Western Blotting of Digested DNA Samples Containing DNA-Protein Crosslinks (DPCs). J. Vis. Exp. (Pending Publication), e200210, doi:10.3791/200210 (2023).

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