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Journal
/
Biochemistry
/
Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction
/
Image Acquisition of Lipid Droplets and Analysis
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Image Acquisition of Lipid Droplets and Analysis
Image Acquisition of Lipid Droplets and Analysis
Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction
DOI:
10.3791/200053-v
•
04:50 min
•
June 02, 2023
•
Karina Garcia
2,3,4
,
André Alves
2,3
,
Teresa M. Ribeiro-Rodrigues
2,3
,
Flávio Reis
2,3
,
Sofia Viana
2,3,4
1
Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine
,
University of Coimbra
,
2
Center for Innovative Biomedicine and Biotechnology (CIBB)
,
University of Coimbra
,
3
Clinical Academic Center of Coimbra (CACC)
,
4
Polytechnic Institute of Coimbra
,
Coimbra Health School
Tags
Lipid Droplets
Image Acquisition
Confocal Microscopy
BODIPY 493/503
DAPI
Laser Scanning
Sequential Scan
20X Objective
10X Objective
40X Objective
Z-stack
Orthogonal Views
3D Imaging
CellProfiler
Image Processing
Identify Primary Objects
Measure Object Intensity
Filter Objects
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