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脳室下帯恩顔：Wholemount染色と上衣の流れ
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow
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脳室下帯恩顔：Wholemount染色と上衣の流れ

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41,202 Views

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14:33 min

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May 06, 2010

DOI:

10.3791/1938-v

DOI:
10.3791/1938-v

•

14:33 min
May 06, 2010

•

9 Views
, , , ,
1Department of Neurosurgery, The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California, San Francisco – UCSF, 2Department of Pathology and Cell Biology,College of Physicians and Surgeons, Columbia University, 3Department of Neuroscience and Neurology,College of Physicians and Surgeons, Columbia University, 4Department of Developmental and Regenerative Biology,Nagoya City University Graduate School of Medical Sciences, 5Center for Motor Neuron Biology and Disease,College of Physicians and Surgeons, Columbia University

Summary

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側脳室の壁は、成体哺乳動物の脳の最大の胚の領域が含まれています。伝統的に、この地域における神経新生の研究は、組織学的分析のための古典的なセクショニングのテクニックに頼ってきた。ここでは、この胚地域の包括的な、EN -顔のビューを提供する代替的なアプローチ、wholemountテクニックを、提示する。

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Cite this Article

Mirzadeh, Z., Doetsch, F., Sawamoto, K., Wichterle, H., Alvarez-Buylla, A. The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow. J. Vis. Exp. (39), e1938, doi:10.3791/1938 (2010).

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