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有丝分裂的siRNA转染后的时间推移成像
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Time-lapse Imaging of Mitosis After siRNA Transfection
DOI:

08:21 min

June 06, 2010

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Chapters

  • 00:00Title
  • 00:45Introduction
  • 01:11siRNA Transfection and Cell Preparation
  • 02:18Microscope Setup and Image Acquisition
  • 05:22Image Processing and Analysis
  • 07:21Effect of Knockdown of Nucleoporin Nup153
  • 07:48Conclusion

Summary

Automatic Translation

在这里,我们描述了一个基本的协议,以图像和量化现场后,转染哺乳动物组织培养细胞的有丝分裂的时机。

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