Journal
/
Biology
/
有丝分裂的siRNA转染后的时间推移成像
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Time-lapse Imaging of Mitosis After siRNA Transfection
Please note that all translations are automatically generated. Click here for the English version.

有丝分裂的siRNA转染后的时间推移成像

English

Automatically Generated

16,628 Views

•

08:21 min

•

June 06, 2010

DOI:

10.3791/1878-v

DOI:
10.3791/1878-v

•

08:21 min
June 06, 2010

•

3 Views
, ,
1Department of Oncological Sciences, Huntsman Cancer Institute,University of Utah, 2Fluorescence Microscopy Core Facility,University of Utah

Summary

Automatically generated

在这里，我们描述了一个基本的协议，以图像和量化现场后，转染哺乳动物组织培养细胞的有丝分裂的时机。

Explore More Videos

Time-lapse ImagingMitosisSiRNA TransfectionCellular OrganizationChromosome DynamicsFluorescence MicroscopyGFP-tagged ProteinsRNAi-based DepletionMitotic Regulatory ProteinAutomated Fluorescence MicroscopeImage SequencesStill-image MontagesMoviesQuantification Of Mitotic StagesMCherry-tagged Histone H2BTime-lapse Experiment Design

Read Article

Cite this Article

Mackay, D. R., Ullman, K. S., Rodesch, C. K. Time-lapse Imaging of Mitosis After siRNA Transfection. J. Vis. Exp. (40), e1878, doi:10.3791/1878 (2010).

Download .ris file

Copy

Share Video

.

Copy