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1Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, 2Broad Institute of Harvard and Massachusetts Institute of Technology, 3Division of Health Sciences and Technology,Massachusetts Institute of Technology, 4Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Department of Mathematics,Harvard University , 5Department of Applied Mathematics,Harvard University , 6Department of Physics,Massachusetts Institute of Technology, 7Department of Systems Biology,Harvard Medical School, 8Department of Biology,Massachusetts Institute of Technology
Chapters
- 00:00Title
- 01:03Introduction
- 02:07Crosslinking, Digestion, Marking of DNA Ends, and Blunt-End Ligation
- 09:46Shearing and Size Selection
- 12:03Biotin Pull-Down and Paired End Sequencing
- 16:38Representative Hi-C Results
- 21:32Conclusion
El método de Hi-C permite la identificación imparcial de todo el genoma de las interacciones entre la cromatina (1). Hi-C parejas ligadura de proximidad y de secuenciación masiva en paralelo. Los datos resultantes se pueden utilizar para estudiar arquitectura genómica a diferentes escalas, los resultados iniciales identificaron características tales como los territorios de cromosomas, la segregación de la cromatina abierta y cerrada, y la estructura de la cromatina en la escala de megabase.
