This content is Free Access.
The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
20,171 Views
•
19:16 min
•
March 17, 2010
DOI:
Tags
Protein ConcentrationKineticsAffinity DeterminationStructure-function AnalysisCysteine Protease InhibitorCystatin BPapainReal-time Label-free AnalysisBiacore X100Point MutationsInteractionCalibration-free Concentration Analysis (CFCA)A280Single-cycle Kinetics (SCK)Association Rate Constant (ka)Affinity (KD)Reliable ResultsInteraction Mechanism
Read Article
Cite this Article
Pol, E. The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis . J. Vis. Exp. (37), e1746, doi:10.3791/1746 (2010).
Copy