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解離成体哺乳類の神経細胞へのDNAの核内マイクロインジェクション
JoVE Journal
Biology
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JoVE Journal Biology
Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons
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解離成体哺乳類の神経細胞へのDNAの核内マイクロインジェクション

日本語

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16,631 Views

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13:39 min

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December 10, 2009

DOI:

10.3791/1614-v

DOI:
10.3791/1614-v

•

13:39 min
December 10, 2009

•

10 Views
, , ,
1Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism (NIAAA),National Institutes of Health (NIH)

Summary

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cDNAの直接核内注入は、有糸分裂後の細胞のための効果的なトランスフェクションの手法です。このメソッドは、単一または複数のcDNA構築物から異種タンパク質の発現の高いレベルを提供し、単一細胞アッセイの様々な生理学的に適切な環境で検討するタンパク質の機能を有効にします。

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Cite this Article

Lu, V. B., Williams, D. J., Won, Y., Ikeda, S. R. Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons. J. Vis. Exp. (34), e1614, doi:10.3791/1614 (2009).

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