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The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF
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Biology
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JoVE Journal Biology
The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF

The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF

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10,324 Views

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05:58 min

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November 18, 2009

DOI:

10.3791/1550-v

DOI:
10.3791/1550-v

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05:58 min
November 18, 2009

•

4 Views
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Summary

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SC1 functions through dual inhibition of Ras- GAP and ERK1. We tested the function of SC1 in supporting mouse ES cell self-renewal in the absence of LIF and showed that SC1 is able to maintain self-renewal of mouse ES cell cultures.

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Xiong, W., Gao, Y., Cheng, X., Martin, C., Wu, D., Yao, S., Kim, M., Liu, Y. The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF. J. Vis. Exp. (33), e1550, doi:10.3791/1550 (2009).

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