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Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

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07:44 min

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March 03, 2023

DOI:

10.3791/65069-v

DOI:
10.3791/65069-v

•

07:44 min
March 03, 2023

•

20 Views
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1Centre for Ecology, Evolution and Environmental Changes and Global Change & Sustainability Institute, Department of Animal Biology, Faculty of Sciences,University of Lisbon

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In this work, a decellularization protocol was optimized to obtain decellularized matrices of fetal mouse skeletal muscle. C2C12 myoblasts can colonize these matrices, proliferate, and differentiate. This in vitro model can be used to study cell behavior in the context of skeletal muscle diseases such as muscular dystrophies.

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Gameiro dos Santos, P., Soares, A. R., Thorsteinsdóttir, S., Rodrigues, G. Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture. J. Vis. Exp. (193), e65069, doi:10.3791/65069 (2023).

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