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Coltura di organoidi intestinali 3D di maialino da cripte epiteliali crioconservate e creazione di monostrati cellulari
JoVE Journal
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JoVE Journal Biyoloji
Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
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Coltura di organoidi intestinali 3D di maialino da cripte epiteliali crioconservate e creazione di monostrati cellulari

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08:19 min

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February 10, 2023

DOI:

10.3791/64917-v

DOI:
10.3791/64917-v

•

08:19 min
February 10, 2023

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6 Views
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1GenPhySE,Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM,Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria,Robert Koch-Institute

Etiketler

Pig Intestinal EpitheliumIntestinal Organoids3D OrganoidsCryopreserved CryptsEpithelial Crypts2D MonolayersNutrient TransportBarrier FunctionHost-microbe InteractionsJejunumDuodenumIleumColonExtracellular MatrixECMCulture MediumPassagingDissociationDMEMRock Inhibitor

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Cite this Article

Mussard, E., Lencina, C., Boudry, G., Achard, C. S., Klotz, C., Combes, S., Beaumont, M. Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers. J. Vis. Exp. (192), e64917, doi:10.3791/64917 (2023).

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