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Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
JoVE Journal
Biology
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JoVE Journal Biology
Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

English

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1,764 Views

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08:19 min

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February 10, 2023

DOI:

10.3791/64917-v

DOI:
10.3791/64917-v

•

08:19 min
February 10, 2023

•

6 Views
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1GenPhySE,Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM,Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria,Robert Koch-Institute

Summary

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Here, we describe a protocol to culture pig intestinal 3D organoids from cryopreserved epithelial crypts. We also describe a method to establish cell monolayers derived from 3D organoids, allowing access to the apical side of epithelial cells.

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Mussard, E., Lencina, C., Boudry, G., Achard, C. S., Klotz, C., Combes, S., Beaumont, M. Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers. J. Vis. Exp. (192), e64917, doi:10.3791/64917 (2023).

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