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In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds – Potential Drug Candidates for Cancer Photopharmacology
JoVE Journal
Kimya
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In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds – Potential Drug Candidates for Cancer Photopharmacology

In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds – Potential Drug Candidates for Cancer Photopharmacology

1,649 Views

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13:17 min

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September 29, 2023

DOI:

10.3791/64902-v

DOI:
10.3791/64902-v

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13:17 min
September 29, 2023

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11 Views
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1Taras Shevchenko National University of Kyiv, 2Bienta/Enamine, 3Karlsruhe Institute of Technology, 4Lumobiotics

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This protocol presents a set of experiments adopted for the evaluation of photoswitchable anticancer peptides, that can be used in the preclinical screening of such compounds. This includes cytotoxicity assessment in 2D and 3D cell cultures, the evaluation of ex vivo (model tissue) photoisomerization efficiency, and in vivo efficacy.

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Horbatok, K., Makhnii, T., Kosach, V., Danko, V., Kovalenko, A., Fatiushchenkov, S., Borysko, P., Pishel, I., Babii, O., Ulrich, A. S., Schober, T., Afonin, S., Komarov, I. V. In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds - Potential Drug Candidates for Cancer Photopharmacology. J. Vis. Exp. (199), e64902, doi:10.3791/64902 (2023).

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