'Dergi'
/
Biyokimya
/
Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
JoVE Journal
Biyokimya
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Biyokimya
Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

DOI:
10.3791/64775-v

•

07:14 min

•

June 16, 2023

•

, , , , , , , ,
1Department of Otolaryngology-Head & Neck Surgery,Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery,University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery,The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory,Johns Hopkins University School of Medicine

'Bölümler'

  • 00:04Introduction
  • 01:03Collection, Preparation, and Extraction of Plasma Sample
  • 03:28ELISA Plate Preparation (Blocking)
  • 04:16Enzyme-Linked Immunosorbent Assay (ELISA)
  • 06:06Results: Effect of Blocking on CGRP Quantification
  • 06:29Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Published data pertaining to calcitonin gene-related peptide (CGRP) concentrations in human plasma are inconsistent. These inconsistencies may be due to the lack of a standardized, validated methodology to quantify this neuropeptide. Here, we describe a validated enzyme-linked immunosorbent assay (ELISA) protocol to purify and quantify CGRP in human plasma.

'Etiketler'

CGRPEnzyme-linked Immunosorbent AssayELISAPlasmaPurificationExtractionQuantificationMigraineBiomarker

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Read Article