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May 05, 2023
DOI:
1Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS,University of Trás-os-Montes and Alto Douro (UTAD), 2Pathology Laboratory,UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, 3Pathology Laboratory,UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão
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使用免疫组织化学方案对异常朊蛋白进行免疫标记需要特定的样品和抗PrP抗体制备方法。本协议描述了表位去掩膜的关键步骤，以确保适当的PrP免疫标记并最大限度地减少非特异性背景染色。此外，这种方法在对朊病毒感染的组织进行免疫组织化学研究时考虑了生物安全措施。
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Orge, L., Lurdes Pinto, M. d., Cristovão, P., Mendonça, P., Carvalho, P., Lima, C., Santos, H., Alves, A., Seixas, F., Pires, I., Gama, A., dos Anjos Pires, M. Detection of Abnormal Prion Protein by Immunohistochemistry. J. Vis. Exp. (195), e64560, doi:10.3791/64560 (2023).
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