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Detection of Abnormal Prion Protein by Immunohistochemistry
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Neuroscience
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JoVE Journal Neuroscience
Detection of Abnormal Prion Protein by Immunohistochemistry

Detection of Abnormal Prion Protein by Immunohistochemistry

English

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2,397 Views

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06:38 min

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May 05, 2023

DOI:

10.3791/64560-v

DOI:
10.3791/64560-v

•

06:38 min
May 05, 2023

•

44 Views
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1Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS,University of Trás-os-Montes and Alto Douro (UTAD), 2Pathology Laboratory,UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, 3Pathology Laboratory,UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão

Summary

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Immunolabeling abnormal prion protein using immunohistochemistry protocols requires specific sample and anti-PrP antibody preparation methodologies. The present protocol describes the key steps in epitope demasking to ensure proper PrP immunolabeling and to minimize non-specific background staining. Also, this approach considers biosafety measures when conducting immunohistochemistry studies with the prion-infected tissues.

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Cite this Article

Orge, L., Lurdes Pinto, M. d., Cristovão, P., Mendonça, P., Carvalho, P., Lima, C., Santos, H., Alves, A., Seixas, F., Pires, I., Gama, A., dos Anjos Pires, M. Detection of Abnormal Prion Protein by Immunohistochemistry. J. Vis. Exp. (195), e64560, doi:10.3791/64560 (2023).

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