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Pulldown-Assay gekoppelt mit Co-Expression in Bakterienzellen als zeiteffizientes Werkzeug zum Testen anspruchsvoller Protein-Protein-Interaktionen
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Biyokimya
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JoVE Journal Biyokimya
Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions
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Pulldown-Assay gekoppelt mit Co-Expression in Bakterienzellen als zeiteffizientes Werkzeug zum Testen anspruchsvoller Protein-Protein-Interaktionen

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07:03 min

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December 23, 2022

DOI:

10.3791/64541-v

DOI:
10.3791/64541-v

•

07:03 min
December 23, 2022

•

19 Views
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1Department of the Control of Genetic Processes, Institute of Gene Biology,Russian Academy of Sciences, 2Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology,Russian Academy of Sciences

Özet

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Hier beschreiben wir eine Methode zur bakteriellen Koexpression von differentiell markierten Proteinen unter Verwendung einer Reihe kompatibler Vektoren, gefolgt von den konventionellen Pulldown-Techniken zur Untersuchung von Proteinkomplexen, die sich in vitro nicht zusammensetzen können.

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Cite this Article

Bonchuk, A., Zolotarev, N., Balagurov, K., Arkova, O., Georgiev, P. Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions. J. Vis. Exp. (190), e64541, doi:10.3791/64541 (2022).

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