Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Artem Bonchuk; Nikolay Zolotarev; Konstantin Balagurov; Olga Arkova; Pavel Georgiev

doi:10.3791/64541

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Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

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生化学
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JoVE Journal 生化学

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

2,365 Views

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07:03 min

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December 23, 2022

DOI:

10.3791/64541-v

DOI:

10.3791/64541-v

•

07:03 min

December 23, 2022

•

19 Views

Artem Bonchuk^1,2, Nikolay Zolotarev¹, Konstantin Balagurov^1,2, Olga Arkova², Pavel Georgiev¹

¹Department of the Control of Genetic Processes, Institute of Gene Biology,Russian Academy of Sciences, ²Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology,Russian Academy of Sciences

概要

Here, we describe a method for the bacterial co-expression of differentially tagged proteins using a set of compatible vectors, followed by the conventional pulldown techniques to study protein complexes that cannot assemble in vitro.

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Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

概要

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