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Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology
Bölümler
- 00:04Introduction
- 00:49Induced Pluripotent Stem Cell-Derived Cardiomyocytes Preparation
- 02:24MEA Recordings
- 04:22Laser-Induced Cell Poration
- 06:28Drug Handling and Application
- 08:01Results: Pharmacological Modulation of the Cardiac Action Potential
- 10:03Conclusion
The combination of laser poration and microelectrode arrays (MEA) allows action potential-like recordings of cultivated primary and stem cell-derived cardiomyocytes. The waveform shape provides superior insight into test compounds' mode of action than standard recordings. It links patch-clamp and MEA readout to further optimize cardio safety research in the future.
