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A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

DOI:
10.3791/64340-v

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08:27 min

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November 30, 2022

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, ,
1School of Chemistry, Chemical Engineering, and Biotechnology,Nanyang Technological University, 2State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering,Zhejiang University of Technology, 3Institute for Digital Molecular Analytics and Science,Nanyang Technological University

Bölümler

  • 00:04Introduction
  • 00:37Cleaning of Nanochip
  • 01:54Generation of Small Unilamellar Vesicles (SUVs)
  • 02:35Formation of the SLB on a Nanochip
  • 03:53Imaging the SLB and the Curvature Sensing Protein Binding on the Nanochip
  • 05:52Results: Protein Curvature Sensing Characterization and Membrane‐Binding Dynamics of Proteins Around the Nanobar
  • 07:43Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, a nanobar-supported lipid bilayer system is developed to provide a synthetic membrane with a defined curvature that enables the characterization of proteins with curvature sensing ability in vitro.

Etiketler

Nanobar-supported Lipid BilayerMembrane Curvature SensingProtein DynamicsNanofabricationPiranha SolutionLipid MixtureSUVsPDMS Chamber

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