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Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae
Bölümler
- 00:05Introduction
- 01:36Tissue Preparation and Fixation
- 02:13Tissue Embedding and Sectioning
- 03:03Vibratome Sectioning and Specimen Clearing
- 04:03Staining Procedure and Microscopy
- 04:49Results: Microscopic Analysis of the Biotrophic Interaction and Gene Expression Regulations Between Plasmodiophora brassicae and Arabidopsis Plants
- 06:06Conclusion
The present protocol describes an optimized method for the histological observation of galls induced by Plasmodiophora brassicae. Vibratome sections of hypocotyls are cleared before fluorescence imaging to study the involvement of transcription factors and phytohormones during disease progression. This protocol overcomes resin embedding limitations, enabling in planta visualization of fluorescent proteins.
