Mice and rats are surgically implanted with remote temperature transponders and then habituated to the testing environment and procedure. Changes in muscle temperature are measured in response to pharmacological or contextual stimuli in the home cage or during prescribed physical activity (i.e., treadmill walking at a constant speed).
Watts, C. A., Haupt, A., Smith, J., Welch, E., Malik, A., Giacomino, R., Walter, D., Mavundza, N., Shemery, A., Caldwell, H. K., Novak, C. M. Measuring Skeletal Muscle Thermogenesis in Mice and Rats. J. Vis. Exp. (185), e64264, doi:10.3791/64264 (2022).