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Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes
JoVE Journal
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Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes

Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes

1,376 Views

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06:35 min

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March 24, 2023

DOI:

10.3791/64169-v

DOI:
10.3791/64169-v

•

06:35 min
March 24, 2023

•

14 Views
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1Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada,The Hebrew University – Hadassah Medical School, 2Shanghai Institute of Immunology, Department of Immunology and Microbiology,Shanghai Jiao Tong University School of Medicine

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Here, we present a protocol to isolate murine intestinal mesenchyme, including telocytes. These can be used for several applications, such as co-culture with mouse or human-derived organoids, to support growth and better reflect the situation in the original tissue.

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Cite this Article

Canella, M., Tan, J., Su, B., Shoshkes-Carmel, M. Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes. J. Vis. Exp. (193), e64169, doi:10.3791/64169 (2023).

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