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相干反斯托克斯拉曼光谱（CARS）在脑切片中成像髓鞘形成的应用
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JoVE Journal Nörobilim
Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices
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相干反斯托克斯拉曼光谱（CARS）在脑切片中成像髓鞘形成的应用

DOI:
10.3791/64013-v

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04:08 min

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July 22, 2022

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, , , ,
1Department of Integrative Biology,Oklahoma State University, 2Department of Physiology and Biophysics,University of Colorado Anschutz, 3Advanced Light Microscopy Core,University of Colorado Anschutz

Bölümler

  • 00:04Introduction
  • 01:19Staining and Imaging of the Sections
  • 02:55Results: Analyzing Brain Tissues Using CARS Imaging
  • 03:34Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

可视化髓鞘形成是许多研究神经系统的研究人员的重要目标。CARS是一种与免疫荧光兼容的技术，可以对组织内的脂质进行天然成像，例如大脑，照亮髓磷脂等特殊结构。

Etiketler

CARSCoherent Anti-Stokes Raman SpectroscopyMyelinationMyelinLipidsCH2 BondsMicroscopyFluorescence ImagingImmunohistochemistryMultiple SclerosisAgingAutismLaser AlignmentForward CARSEpi CARSConfocal Imaging

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