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Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices
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JoVE Revista Neurociencias
Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices

Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices

2,332 Views

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04:08 min

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July 22, 2022

DOI:

10.3791/64013-v

DOI:
10.3791/64013-v

•

04:08 min
July 22, 2022

•

7 Views
, , , ,
1Department of Integrative Biology,Oklahoma State University, 2Department of Physiology and Biophysics,University of Colorado Anschutz, 3Advanced Light Microscopy Core,University of Colorado Anschutz

Summary

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Visualizing myelination is an important goal for many researchers studying the nervous system. CARS is a technique that is compatible with immunofluorescence that can natively image lipids within tissue such as the brain illuminating specialized structures such as myelin.

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Cite this Article

McCullagh, E. A., Poleg, S., Stich, D., Moldovan, R., Klug, A. Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices. J. Vis. Exp. (185), e64013, doi:10.3791/64013 (2022).

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