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Examining the Effect of Pesticides on Caenorhabditis elegans Neurons
Bölümler
- 00:04Introduction
- 00:25Preparation of Pesticide-Coated Petri Plates
- 01:16Adding Nematodes to Pesticide-Coated Plates
- 01:57Preparing Microscope Slides for Wet Mounts
- 02:41Mounting Live Nematodes to Microscope Slides
- 03:29Fluorescent Microscopy
- 04:23Results: Effects of Pesticide on Neuron Morphology
- 04:45Conclusion
Young adult Caenorhabditis elegans nematodes are exposed to different concentrations of commercial pesticides or other toxicants for 2-24 h. Then, different neurons can be visualized using fluorescent-expressing strains. This paper demonstrates how to expose nematodes to pesticides and assess neuron damage.
