Dergi
/
İmmünoloji ve Enfeksiyon
/
Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice
JoVE Journal
İmmünoloji ve Enfeksiyon
Author Produced
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice

Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice

DOI:
10.3791/63695-v

•

14:10 min

•

July 29, 2022

•

, , ,
1Department of Biochemistry,Indian Institute of Science, 2Center for BioSystems Science and Engineering,Indian Institute of Science, 3Undergraduate Program,Indian Institute of Science

Bölümler

  • 00:03Introduction
  • 02:20Culture Preparation of Salmonella Typhimurium
  • 05:15Mice and Infections
  • 05:56CFU Assessment of Organs
  • 09:06Flow Cytometric Analysis of Various Immune Cell Populations in Peritoneal Exudate
  • 11:56Sonuçlar
  • 13:22Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol describes the induction of Gram-negative monobacterial sepsis in a mouse model system. The model is useful in investigating the inflammatory and lethal host responses during sepsis.

Etiketler

Salmonella TyphimuriumSeptic PeritonitisSepsisMice ModelGram-negative InfectionBacterial BurdenBSL-2LB BrothSalmonella Shigella Agar

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Colon Ascendens Stent Peritonitis (CASP) – a Standardized Model for Polymicrobial Abdominal Sepsis

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

Chronic Salmonella Infection Induced Intestinal Fibrosis

Read Article