Dergi
/
Nörobilim
/
Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

2,985 Views

•

09:36 min

•

June 23, 2022

DOI:

10.3791/63483-v

DOI:
10.3791/63483-v

•

09:36 min
June 23, 2022

•

17 Views
, , ,
1Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”,University of Belgrade Faculty of Biology

Özet

Automatically generated

We present here a protocol on how to prepare primary cultures of glial cells, astrocytes, and microglia from rat cortices for time-lapse video imaging of intracellular Ca2+ for research on pathophysiology of amyotrophic lateral sclerosis in the hSOD1G93A rat model.

Explore More Videos

Primary Astrocyte CulturePrimary Microglia CultureCalcium SignalingAmyotrophic Lateral SclerosisNeuroinflammatory DiseasesPersonalized MedicineCell IsolationCell CultureDMEMFBSAntibiotics

Read Article

Cite this Article

Milićević, K., Korenić, A., Milošević, M., Andjus, P. R. Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis. J. Vis. Exp. (184), e63483, doi:10.3791/63483 (2022).

Download .ris file

Copy

Share Video

.

Copy