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Isolation and Characterization of Cyanobacterial Extracellular Vesicles
1Department of Biological Sciences,Wellesley College, 2Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional, Agroalimentario,Universidad de Córdoba, 3i3S – Instituto de Investigação e Inovação em Saúde,Universidade do Porto, 4IBMC – Instituto de Biologia Molecular e Celular,Universidade do Porto, 5MCbiology Doctoral Program, ICBAS – Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto, 6Departamento de Biologia, Faculdade de Ciências,Universidade do Porto
Bölümler
- 00:05Introduction
- 00:38Concentrating the Vesicle Sample
- 02:58Vesicle Isolation and Purification
- 04:47Characterization of the Isolated Vesicles Using Transmission Electron Microscopy
- 05:22Characterization of the Isolated Vesicles Using Nanoparticle Tracking Analysis
- 06:38Results: An Overview of the Cyanobacterial Vesicle Isolation
- 07:42Conclusion
The present protocol providesdetailed descriptions for isolation, concentration,and characterization of extracellular vesicles from cyanobacterial cultures. Approaches for purifying vesicles from cultures at different scales, trade-offs among methodologies, and considerations for working with field samples are also discussed.
