Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Optrode Array para modulación optogenética simultánea y grabación neuronal eléctrica
1Department of Electronic and Electrical Engineering,Ewha Womans University, 2Graduate Program in Smart Factory,Ewha Womans University, 3Elektrik ve Bilgisayar Mühendisliği Bölümü,Seoul National University, 4Graduate School of Engineering Practice,Seoul National University, 5Department of Brain and Cognitive Sciences,Ewha Womans University
Bölümler
- 00:04Introduction
- 01:13Preparation of an Optrode Array
- 01:39Presurgical Preparation of Animal and Implantation Surgery
- 03:45Setting Parameters and Conducting Optogenetic Stimulation, Electrophysiological Recording, and Data Analysis
- 04:55Results: Electrophysiological Recording
- 05:40Conclusion
Aquí, presentamos el método de fabricación de un sistema optrode con fibras ópticas para la entrega de luz y una matriz de electrodos para la grabación neuronal. Los experimentos in vivo con ratones transgénicos que expresan canalrodopsina-2 muestran la viabilidad del sistema para la estimulación optogenética simultánea y el registro electrofisiológico.
