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福斯特共振能量转移映射：阐明全球结构特征的新方法
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JoVE Journal Biyokimya
Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
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福斯特共振能量转移映射：阐明全球结构特征的新方法

2,302 Views

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07:09 min

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March 16, 2022

DOI:

10.3791/63433-v

DOI:
10.3791/63433-v

•

07:09 min
March 16, 2022

•

2 Views
, ,
1Molecular Biology and Biochemistry Department,Wesleyan University, 2Molecular Biophysics Program,Wesleyan University

Özet

Automatically generated

该研究详细介绍了FRET映射的方法，包括标记位点的选择，染料的选择，采集和数据分析。该方法可有效确定蛋白质系统中的结合位点、构象变化和动态运动，如果与现有的3-D结构信息结合使用，这种方法最有用。

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Keywords Extracted From The Given Text:FRET MappingLigand Binding SitesSubunit OrientationConformational ChangesDynamic MotionsBiomolecular SystemLabeling SitesR0 ValuesDonor DyeAcceptor DyeSpectrofluorometerEmission ScanExcitation Wavelength

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Cite this Article

Northrop, J., Oliver, D. B., Mukerji, I. Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features. J. Vis. Exp. (181), e63433, doi:10.3791/63433 (2022).

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