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Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

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07:09 min

March 16, 2022

DOI:

07:09 min
March 16, 2022

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Summary

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The study details the methodology of FRET mapping including the selection of labeling sites, choice of dyes, acquisition, and data analysis. This methodology is effective at determining binding sites, conformational changes, and dynamic motions in protein systems and is most useful if performed in conjunction with existing 3-D structural information.

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