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Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
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Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
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March 16, 2022
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The study details the methodology of FRET mapping including the selection of labeling sites, choice of dyes, acquisition, and data analysis. This methodology is effective at determining binding sites, conformational changes, and dynamic motions in protein systems and is most useful if performed in conjunction with existing 3-D structural information.
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Northrop, J., Oliver, D. B., Mukerji, I. Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features. J. Vis. Exp. (181), e63433, doi:10.3791/63433 (2022).
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