Dergi
/
Nörobilim
/
Protocolo basado en microdisección láser para el análisis LC-MS/MS del perfil proteómico de gránulos de neuromelanina
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
Please note that all translations are automatically generated. Click here for the English version.

Protocolo basado en microdisección láser para el análisis LC-MS/MS del perfil proteómico de gránulos de neuromelanina

2,000 Views

•

07:35 min

•

December 16, 2021

DOI:

10.3791/63289-v

DOI:
10.3791/63289-v

•

07:35 min
December 16, 2021

•

32 Views
, , , , , , , , ,
1Medizinisches Proteom-Center, Medical Faculty,Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI),Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy,University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research,University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy,University Hospital of Wuerzburg, University of Wuerzburg

Özet

Automatically generated

Aquí se presenta un protocolo robusto para aislar gránulos de neuromelanina del tejido humano post-mortem substantia nigra pars compacta mediante microdisección láser. Este protocolo revisado y optimizado minimiza enormemente el tiempo requerido para la recolección de muestras, reduce la cantidad de muestra requerida y mejora la identificación y cuantificación de proteínas mediante análisis LC-MS / MS.

Explore More Videos

Laser MicrodissectionProteomic ProfileNeuromelanin GranulesMass SpectrometryProteomic AnalysisDisease MechanismsRobo StageField Of View AnalysisLaser SettingsSample CollectionTriptic DigestionHPLC-MS AnalysisProteomic Raw Data Analysis

Read Article

Cite this Article

Wulf, M., Barkovits-Boeddinghaus, K., Sommer, P., Schork, K., Eisenacher, M., Riederer, P., Gerlach, M., Kösters, S., Eggers, B., Marcus, K. Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules. J. Vis. Exp. (178), e63289, doi:10.3791/63289 (2021).

Download .ris file

Copy

Share Video

.

Copy