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Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
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JoVE Revista Neurociencias
Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

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07:35 min

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December 16, 2021

DOI:

10.3791/63289-v

DOI:
10.3791/63289-v

•

07:35 min
December 16, 2021

•

32 Views
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1Medizinisches Proteom-Center, Medical Faculty,Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI),Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy,University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research,University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy,University Hospital of Wuerzburg, University of Wuerzburg

Summary

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A robust protocol is presented here for isolating neuromelanin granules from human post-mortem substantia nigra pars compacta tissue via laser microdissection. This revised and optimized protocol massively minimizes the required time for sample collection, reduces the required sample amount, and enhances the identification and quantification of proteins by LC-MS/MS analysis.

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Laser MicrodissectionProteomic ProfileNeuromelanin GranulesMass SpectrometryProteomic AnalysisDisease MechanismsRobo StageField Of View AnalysisLaser SettingsSample CollectionTriptic DigestionHPLC-MS AnalysisProteomic Raw Data Analysis

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Wulf, M., Barkovits-Boeddinghaus, K., Sommer, P., Schork, K., Eisenacher, M., Riederer, P., Gerlach, M., Kösters, S., Eggers, B., Marcus, K. Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules. J. Vis. Exp. (178), e63289, doi:10.3791/63289 (2021).

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