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体外 使用表达β半乳糖苷酶的寄生虫针对 克氏锥虫 的所有生命周期阶段进行药物筛选
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JoVE Journal Biyokimya
In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase
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体外 使用表达β半乳糖苷酶的寄生虫针对 克氏锥虫 的所有生命周期阶段进行药物筛选

3,046 Views

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08:48 min

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November 05, 2021

DOI:

10.3791/63210-v

DOI:
10.3791/63210-v

•

08:48 min
November 05, 2021

•

3 Views
, , , , ,
1Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas,Universidad Nacional de Rosario (UNR), 2Instituto de Biología Molecular y Celular de Rosario (IBR),CONICET, 3Instituto de Investigaciones Biomédicas (INBIOMED),Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

Özet

Automatically generated

我们描述了一种高通量比色测定法，用于测量恰加斯病病原体 克氏锥虫三个生命周期阶段的β半乳糖苷酶活性。该测定可用于以简单，快速和可重复的方式鉴定杀锥虫化合物。

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Trypanosoma CruziIn Vitro Drug ScreeningHigh-throughput Colorimetric AssayParasite Life Cycle StagesEpimastigotesTrypomastigotesVero CellsBeta-galactosidaseChagas DiseaseDrug Discovery

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Cite this Article

Alonso, V. L., Manarin, R., Perdomo, V., Gulin, E., Serra, E., Cribb, P. In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase. J. Vis. Exp. (177), e63210, doi:10.3791/63210 (2021).

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