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In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase
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Bioquímica
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JoVE Journal Bioquímica
In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase

In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase

DOI:
10.3791/63210-v

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08:48 min

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November 05, 2021

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, , , , ,
1Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas,Universidad Nacional de Rosario (UNR), 2Instituto de Biología Molecular y Celular de Rosario (IBR),CONICET, 3Instituto de Investigaciones Biomédicas (INBIOMED),Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

Capítulos

  • 00:04Introduction
  • 00:38Trypanosoma cruzi Epimastigotes Culture Preparation
  • 01:58Trypanosoma cruzi Amastigote Culture Preparation
  • 03:09Trypanosoma cruzi Trypomastigote Culture Preparation
  • 04:50β-Galactosidase Assay
  • 07:59Results: Results of β-Galactosidase Assay
  • 08:23Conclusion

Summary

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We describe a high-throughput colorimetric assay measuring β-galactosidase activity in three life cycle stages of Trypanosoma cruzi, the causative agent of Chagas disease. This assay can be used to identify trypanocidal compounds in an easy, fast, and reproducible manner.

Tags

Trypanosoma CruziIn Vitro Drug ScreeningHigh-throughput Colorimetric AssayParasite Life Cycle StagesEpimastigotesTrypomastigotesVero CellsBeta-galactosidaseChagas DiseaseDrug Discovery

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