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模拟人血脑屏障的三重培养细胞系统
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

模拟人血脑屏障的三重培养细胞系统

DOI:

09:21 min

November 30, 2021

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Bölümler

  • 00:04Introduction
  • 01:23Seeding of Astrocytes in the Wells
  • 02:50Seeding of Pericytes on the Reverted Insert Filters
  • 04:35Seeding of Endothelial Cells on the Insert Filters
  • 06:00Maintenance of the Triple Cell Culture for the Induction of the BBB Properties
  • 06:35Results: Properties of the Triple Culture BBB Model and the Comparison of BLEC Gene Expression and Protein Level of Distinctive Markers in the Triple Culture Model with the Co‐culture BBB Model
  • 08:49Conclusion

Özet

Otomatik Çeviri

该协议描述了一种建立人血脑屏障(BBB)体 模型的方法。内皮细胞和周细胞接种在插入过滤器(血室)的每一侧,星形胶质细胞接种在底孔(脑隔室)中。所表征的模型用于纳米颗粒输运实验。

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